The contribution of recombinant DNA

Page Introduction 2 Structures of genes and mRNA molecules 2 Structure of genes 2 Sequences of promoters and enhancers 3 Signals for terminating RNA synthesis 5 Structure of mRNA molecules 5 Structure of genes coding for reproductive polypeptide hormones and hypothalamic releasing factors 7 Structure of gonadotrophin genes 7 Structure of placental lactogen, prolactin and growth hormone genes 10 Structure of the LHRH gene 12 Inhibin, activin and M\l=u"\llerianduct-inhibiting hormone 13 Localization of gene products by in-situ hybridization 16 Preand post-translational processing 18 Pretranslational processing: differential splicing of RNA 18 Post-translational processing 18 Steroidogenic enzymes and steroid sulphatase 20 Steroid hormone receptors 22 Steroid receptor primary structure: amino acid sequences derived from cloned DNA 23 Steroid hormone receptors and oncogenesis 26 Mechanism of binding of receptors to DNA\p=m-\thefinger hypothesis 27 DNA sequences recognized by steroid receptors in eukaryotic cells 28 Fertilization and early development 30 Expression of the embryonic genome 30 Pattern formation in early development 32 Oncogenes and heat-shock proteins 33 Oncogenes 33 Heat-shock proteins 38 The future 39 Appendix 1 : recombinant DNA techniques and procedures 40 Appendix 2: glossary of terms 45 Appendix 3: bibliography (textbooks and practical manuals) 47 References 47